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The distribution and some properties of trehalase (α,α-trehalose 1-D-glucohydrolase, EC 3.2.1.28) in Ascaris suum intestine has been studied. α,α-Trehalase was solubilized from the intestinal mitochondria of Ascaris suum with 0.5% Triton X-100 and 0.2% sodium deoxycholate. Solubilized supernatant was purified with solid ammonium sulfate, passed through Sephadex G-200 and chromatographed on the DEAE-cellulose column. The activity of trehalase was measured by the method of Lapp and Mason. The results obtained were as follows; 1. The activity of trehalase in Ascaris suum intestine was 41,809 units/g. This enzyme was distributed in the cytosolic, mitochondrial and nucleic fraction. About 68% of the total activity was found in the cytosolic fraction, 26% in the nucleic fraction. 2. Particulate form of trehalase was isolated from intestinal mitochondria and purified approximately 90-fold. 3. This enzyme was highly specific for trehalose as substrate. 4. The optimum pH of the trehalase was 5.5 in phosphate buffer. Acetate buffer, however, shifted the optimum pH to 4.8. 5. The apparent molecular weight of the purified enzyme was estimated to be 102,300 by Sephadex G-200 gel filtration.
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